Synthetic Neurobiology Group

Ed Boyden, Ph.D., Principal Investigator

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Protocols and Reagents

Optogenetic molecular reagents enabling control of the activity of targeted neurons and biological functions with light

  • ArchT: Archaerhodopsin from Halorubrum sp. TP009: genetically-encoded reagent for high-performance yellow/green-light neural silencing, 3.5x more light sensitive than Arch

  • Arch: Archaerhodopsin from H. sodomense: genetically-encoded reagent for high-performance yellow/green-light neural silencing

  • Mac: opsin from L. maculans: genetically-encoded reagent for blue/green-light neural silencing

  • ChR2-2A-Halo: Channelrhodopsin-halorhodopsin gene fusions: bi-directional neural control with two different colors of light

  • Halo/NpHR: Halorhodopsin from N. pharaonis: genetically-encoded reagent for yellow-light neural hyperpolarization

  • ChR2: Channelrhodopsin-2 from C. reinhardtii: genetically-encoded reagent for blue-light neural activation

  • Accessory reagents for optogenetics

  • Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Optogenetic hardware and systems for control of neural circuits and biological functions with light

  • Versatile, but Manually-Assembled, Optical Fiber/Laser System for In Vivo (Multicolor) Light Delivery for Brain Neuromodulation

  • Very Simple Off-The-Shelf Laser and Viral Injector Systems for In Vivo Optical Neuromodulation

Home » Protocols and Reagents » Optogenetic molecular reagents enabling control of the activity of targeted neurons and biological functions with light

Optogenetic molecular reagents enabling control of the activity of targeted neurons and biological functions with light

ArchT: Archaerhodopsin from Halorubrum sp. TP009: genetically-encoded reagent for high-performance yellow/green-light neural silencing, 3.5x more light sensitive than Arch

Arch: Archaerhodopsin from H. sodomense: genetically-encoded reagent for high-performance yellow/green-light neural silencing

Mac: opsin from L. maculans: genetically-encoded reagent for blue/green-light neural silencing

ChR2-2A-Halo: Channelrhodopsin-halorhodopsin gene fusions: bi-directional neural control with two different colors of light

Halo/NpHR: Halorhodopsin from N. pharaonis: genetically-encoded reagent for yellow-light neural hyperpolarization

ChR2: Channelrhodopsin-2 from C. reinhardtii: genetically-encoded reagent for blue-light neural activation

Accessory reagents for optogenetics

Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Copyright 1995-2012, Ed Boyden esb@media.mit.edu